N NUCELOTIDE SEQUENCE SNAPGENE VIEWER CODE

SVBV-infected cells contain cytoplasmic inclusion bodies, typical of those produced by other caulimoviruses.

N NUCELOTIDE SEQUENCE SNAPGENE VIEWER CODE

SVBV-US has a 7.8В kb circular dsDNA genome with one single-stranded discontinuity on each DNA strand, with seven open reading frames with the potential to code for seven proteins. So far, the only complete nucleotide sequence of SVBV is for an isolate from the United States (accession number: X97304). The virus contains a double-stranded (ds) DNA genome and is classified in the genus Caulimovirus of the family Caulimoviridae. SVBV is a plant virus characterized by the equiaxed shape and 40 to 45В nm particles. However, cultivated strawberry plants are symptomless when infected with SVBV alone, while co-infection of SVBV with other strawberry viruses, strawberry mottle virus and strawberry mild yellow edge virus, has the potential to cause serious symptoms in strawberry plants. virginiana, are bands of yellowing along the main leaf veins and partial reduction in growth potential and twisting of leaflets. Typical symptoms observed in infected indicator plants, F. SVBV could only be detected on Fragaria species in naturally occurring infections. The virus was shown to be transmissible by grafting or by several aphid species in a semi-persistent manner, and distributed world-wide on cultivated strawberries. The first report of strawberry vein banding virus (SVBV) was in 1955, which has been followed by additional research in Europe and America. This is the first report describing an infectious clone of SVBV-CN, and that vacuum infiltration can be potentially used as a new and highly efficient means for inoculation of strawberry plants. Agroinfiltration of strawberry plants using an infectious clone of SVBV-CN resulted in symptoms typically found in infected strawberries from Shenyang city of Liaoning province in China. The complete nucleotide sequence of SVBV from a naturally infected strawberry was determined.

This compares to an infection rate of 20вЂ“40В % in 8вЂ“9 weeks post-inoculation using syringe-inoculation. Furthermore, strawberry plants inoculated with the infectious clone using vacuum infiltration developed symptoms with a very high infection rate of 86вЂ“100В % in 4-5 weeks post-inoculation. This suggests that the SVBV-CN infectious clone can recapitulate the symptoms observed in naturally infected strawberries, and therefore is likely the causal agent of the original disease observed in strawberries. Agrobacterium-mediated inoculation of Fragaria vesca with an infectious clone of SVBV-CN results in systemic infection with distinct symptoms of yellowing bands along the main leaf veins.

SVBV-CN clustered together with SVBV-US, whereas other caulimoviruses formed a separate branch. Two major clades were identified based on phylogenetic analysis of the complete genome sequences of caulimoviruses. Sequence analysis revealed a complete genome of 7864 nucleotides (nts) that indicated SVBV-CN was most closely related to SVBV from the United States (SVBV-US) with a sequence similarity of 85.8В %. Sachinoka) sample found in Shenyang city of Liaoning province. The complete genome of an SVBV Chinese isolate (SVBV-CN) was isolated and cloned from a naturally infected strawberry ( FragariaвЂ‰Г—вЂ‰ananassa cv. It is one of the most economically important diseases in Asiatic, European and North American strawberry-growing areas. Infected strawberry plants exhibit mild vein-banding symptoms and chlorosis along the veins.

Strawberry vein banding virus (SVBV) is a double-stranded DNA plant virus, which has been found in North America, Australia, Brazil, Japan, Europe and several provinces of China.

N nucelotide sequence snapgene viewer